Human Heredity Principles And Issues 11th Edition by Michael Cummings – Test Bank

True / False

1. Plasmids are short sequences of DNA used as primers in PCR.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-3 Cloning Genes Is a Multistep Process
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-3-2 – Outline the three requirements for DNA cloning, and explain the role each plays in the construction of DNA clones.

2. Repeating the sequence of the polymerase chain reaction doubles the number of DNA fragments each time.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-6 A Revolution in Cloning: The Polymerase Chain Reaction
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-6-1 – Outline the polymerase chain reaction (PCR) and list its advantages over host-cell cloning.

3. Adult cells cannot be used as the source of nuclei for cloning animals.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-2 What Are Clones?
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-2-1 – Describe the methods by which clones can be produced and summarize the effects of cloning technology on agriculture.

4. During gel electrophoresis, DNA fragments are separated by size.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-7 Analyzing Cloned Sequences
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-7-1 – Describe the technique of Southern blots, and summarize its applications in genetic technology.

5. The basic method of DNA sequencing is the Sanger method.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-7 Analyzing Cloned Sequences
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-7-2 – Illustrate the Sanger method of DNA sequencing, and summarize the applications of this technique.

6. The human genome contains approximately 40,000 genes.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-5 Finding a Specific Gene in a Library
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-5-1 – Examine how library screening can identify a specific gene in a genomic library.

7. Edwin Southern and his colleagues started the field of genomics.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-7 Analyzing Cloned Sequences
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-7-3 – Summarize the history of DNA sequencing.

8. To interpret microarray data, it is necessary to understand the significance of the color of each dot within the array.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-8 DNA Microarrays Are Used to Analyze Gene Expression
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-8-1 – Outline the steps of a DNA microarray experiment, and describe how this technique is able to analyze the expression of thousands of genes in a single experiment.

9. Growth hormones given to dairy cows would still be necessary to stimulate milk production in a cloned animal.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-1 Making Choices About Biotechnology
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-1-1 – Debate the advantages and disadvantages of animal cloning, as well as the consumption of products produced by cloned animals.

10. A collection of clones containing all the DNA sequences carried by an individual is called a genomic library.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-4 Cloned Libraries
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-4-1 – Differentiate between genomic libraries, chromosomal libraries, and expressed sequence libraries.

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	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-3 Cloning Genes Is a Multistep Process
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-3-2 – Outline the three requirements for DNA cloning, and explain the role each plays in the construction of DNA clones.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-6 A Revolution in Cloning: The Polymerase Chain Reaction
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-6-1 – Outline the polymerase chain reaction (PCR) and list its advantages over host-cell cloning.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-2 What Are Clones?
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-2-1 – Describe the methods by which clones can be produced and summarize the effects of cloning technology on agriculture.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-7 Analyzing Cloned Sequences
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-7-1 – Describe the technique of Southern blots, and summarize its applications in genetic technology.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-7 Analyzing Cloned Sequences
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-7-2 – Illustrate the Sanger method of DNA sequencing, and summarize the applications of this technique.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-5 Finding a Specific Gene in a Library
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-5-1 – Examine how library screening can identify a specific gene in a genomic library.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-7 Analyzing Cloned Sequences
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-7-3 – Summarize the history of DNA sequencing.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-8 DNA Microarrays Are Used to Analyze Gene Expression
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-8-1 – Outline the steps of a DNA microarray experiment, and describe how this technique is able to analyze the expression of thousands of genes in a single experiment.

	a.	True
	b.	False

ANSWER:	False
DIFFICULTY:	Bloom’s: Understand
REFERENCES:	13-1 Making Choices About Biotechnology
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-1-1 – Debate the advantages and disadvantages of animal cloning, as well as the consumption of products produced by cloned animals.

	a.	True
	b.	False

ANSWER:	True
DIFFICULTY:	Bloom’s: Remember
REFERENCES:	13-4 Cloned Libraries
LEARNING OBJECTIVES:	HUHE.CUMM.16.13-4-1 – Differentiate between genomic libraries, chromosomal libraries, and expressed sequence libraries.

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